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KMID : 0379520020180040363
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한국독성학회지
2002 Volume.18 No. 4 p.363 ~ p.367
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Inhibition of IgM Secretion in Murine B Cell Lymphoma by Hydrogen Peroxide
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Jang Eun-Jung
Jo Sung-Kee
Yoo Byung-Sun
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Abstract
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Reactive of gen species (ROS) contribute to several cellular function and are involved in the regulation of signal transduction, gene expression, and proliferation. In the present study, we investigated the effect of H_2O_2 treatment on IgM secretion in LPS-stimulated murine B Iymphoma, CH12.LX. Cells were treated directly With H_2O_2 and stimulated with LPS. H_2O_2 treatment during 72 h time period inhibited IgM secretion in LPS-stimulated CH12.LX cells in a dose- and time-dependent manners. After treatment with 50 mutextrm{M} H_2O_2 during 72 h time period, the level of IgM in LPS-stimulated CH12.LX cells was markedly decreased, whereas cell viability was not significantly changed. Addition of H_2O_2 concomitantly with LPS, or 12 h post-LPS stimulation, produced a significant inhibition of IgM secretion, Whereas inhibitory effect of H_2O_2 on IgM secretion was not observed when added 24 h after LPS stimulation. These findings suggest that H_2O_2 can inhibit the secretion of IgM in LPS-stimulated CH15.LX cells, and may alter the events necessary for terminal B cell differentiation.
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KEYWORD
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H_2O_2, IgM, 3 Cell lymphoma
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